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Loss of the Otx2-Binding Site in the Nanog Promoter Affects the Integrity of Embryonic Stem Cell Subtypes and Specification of Inner Cell Mass-Derived Epiblast

Loss of the Otx2-Binding Site in the Nanog Promoter Affects the Integrity of Embryonic Stem Cell Subtypes and Specification of Inner Cell Mass-Derived Epiblast

• Otx2 binds to the promoter/enhancer region of Oct4, Sox2, and Nanog in ESCs and EpiSCs • Otx2 binding to the Nanog promoter helps maintain the integrity of ESC compartments • Loss of this Otx2-binding site induces primed-like features in ESCs • Otx2 regulation of Nanog contributes to ICM differentiation of the epiblast Mouse embryonic stem cells (ESCs) and the inner cell mass (ICM)-derived epiblast exhibit naive pluripotency. ESC-derived epiblast stem cells (EpiSCs) and the postimplantation epiblast exhibit primed pluripotency. Although core pluripotency factors are well-characterized, additional regulators, including Otx2, recently have been shown to function during the transition from naive to primed pluripotency. Here we uncover a role for Otx2 in the control of the naive pluripotent state. We analyzed Otx2-binding activity in ESCs and EpiSCs and identified Nanog, Oct4, and Sox2 as direct targets. To unravel the Otx2 transcriptional network, we targeted the strongest Otx2-binding site in the Nanog promoter, finding that this site modulates the size of specific ESC-subtype compartments in cultured cells and promotes Nanog expression in vivo, predisposing ICM differentiation to epiblast. Otx2-mediated Nanog regulation thus contributes to the integrity of the ESC state and cell lineage specification in preimplantation development.

CNR Science 22 Jun 2016
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March of the motes

March of the motes

An unseen silicon army is gathering information on Earth and its inhabitants. It’s not an alien reconnaissance mission: smart dust is finally hitting the streets, says Anil Ananthaswamy

Redazione Science 27 Apr 2013
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E Mario Monti perde la sobrietĂ  su facebook

E Mario Monti perde la sobrietĂ  su facebook

le informazioni raccolte in questa notizia sono a cura di Alfredo d’Ecclesia, tuttavia la redazione ha eseguito un controllo ricavando un PDF estrapolato dalla pagina di Facebook aperta da un gruppo che intende sostenere l’on Mario Monti. Il documento PDF riporta parte delle notifiche relative a questa news (E’ stata eseguita una visualizzazione della pagina e di seguito congelato nel formato PDF che trovate in allegato). Riproduzione Vietata e’ consentita la condivisione.

Alfredo_d_Ecclesia Voiceofthecitizen 22 Jan 2013
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